Detection of Mycobacterium Paratuberculosis using polymerase chain reaction (PCR) in cow raw milk samples in shahre-kord
Authors
Abstract:
Abstract Background and objectives: Paratuberculosis or John's disease is a chronic infectious disease of ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). It results in major economic losses to dairy farm of all over the world and it is the agent causing crohn's disease. The aim of this study was to detect the MAP using PCR in raw-milk samples of cows in shahre-kord. Material and Methods: In this cross–sectional study, 100 raw milk samples of cows were collected from both industrial and semi -industrial farms in shahre-kord. The DNA of all Samples was isolated by MAP, using PCR method. Results: The results Show that only three (3%) Samples were positive for Mycobacterium avium subsp. paratuberculosis. Conclusion: Based on our results, Milk -PCR was useful for detection of MAP in milk samples. Key words: Mycobacterium paratuberculosis, milk, polymerase chain reaction.
similar resources
Detection of Mycobacterium avium subsp. paratuberculosis in Cow Milk Using Culture and PCR methods
Mycobacterium avium subspecies paratuberculosis (MAP) is the cause of John’s disease also called paratuberculosis. This is economically one of the important infectious diseases in cattle and ruminant husbandry. This disease is manifested as granulomatosis entrocolitis, lymphadenitis and inflammation local lymphatic vessels. The typical sign of this disease is progressive loss of weight. Cons...
full textDetection of Chicken Meat Adulteration in Raw Hamburger Using Polymerase Chain Reaction
Background: Detection of food adulteration is an important issue from aspects of food control and food regulation. This study aimed to detect adulteration of chicken meat in raw hamburger using species specific Polymerase Chain Reaction (PCR). Methods: Raw hamburgers including 42 handmade and 48 industrial samples were collected from 90 restaurants and supermarkets. Following genomic DNA extra...
full textdetection of mycobacterium avium subsp. paratuberculosis in cow milk using culture and pcr methods
mycobacterium avium subspecies paratuberculosis (map) is the cause of john’s disease also called paratuberculosis. this is economically one of the important infectious diseases in cattle and ruminant husbandry. this disease is manifested as granulomatosis entrocolitis, lymphadenitis and inflammation local lymphatic vessels. the typical sign of this disease is progressive loss of weight. cons...
full textRAPID DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN CLINICAL SPECIMENS BY POLYMERASE CHAIN REACTION
We investigated the use of DNA amplification by polymerase chain reaction (peR) for detection of Mycobacterium tuberculosis in 300 patients who were suspected of having pulmonary tuberculosis and compared the results with culture results which were performed in parallel with PCR. Two-thirds of each sample was processed for smear and culture by standard methods and one-third was prepared fo...
full textMolecular detection of Ehrlichia spp. in blood samples of dogs in southern Iran using polymerase chain reaction
Ehrlichiosis is a zoonotic disease which has been reported from some regions of Iran. This study was aimed to determine the presence and prevalence of ehrlichiosis in suspected dogs referred to the Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran using polymerase chain reaction(PCR). Blood samples were collected from 98 suspected dogs with at least one of the five following findi...
full textisolation of enteropathogenic escherichia coli (epec) from raw milk in kermanshah by polymerase chain reaction (pcr)
background infectious diarrhoeal diseases cause major problems throughout the world and are responsible for considerable morbidity and mortality. enteropathogenic escherichia coli (epec) may cause infantile diarrhoea among children in developing countries. objectives the aim of this study was to define the prevalence of epec strains in raw milk samples. materials and methods raw milk samples co...
full textMy Resources
Journal title
volume 3 issue 1
pages 0- 0
publication date 2009-04
By following a journal you will be notified via email when a new issue of this journal is published.
No Keywords
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023